2009•Food Science and Technology InternationalRequires access

Comparison of methods in determination of polysaccharide in Lycium barbarum L.

Xiaojuan Fang

Abstract

Objective: To compare difference in determination content between phenol -vitriolic colorimetry method and anthrone sulphuric acid colrimetry method for polysaccharides in Lycium barbarum. Methods: Above two methods were employed to determine content of polysaccharides by UV spectrophotography. Results: The determination value of anthrone-sulphuric acid colorimetry method is high than the phenol-vitriolic colorimetry method, and its values dissimilarity have statistical significance between two methods.Conclusion: The result of phenol-vitriolic colorimetry method is more reliable and accurate, it can be considered as the optimum assay method for polysaccharides in Lycium barbarum.

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Key concepts: Colorimetry, Lycium, Polysaccharide, Chemistry, Anthrone, Phenol, Chromatography, Biochemistry