Effect of Epmedin C on Estrogen in Ovariectomized Mice

Objective:To observe the effect of Epmedin C of estrogen in ovariectomized mice.Methods: Anesthetized with 0.4%pentobarbital sodium,mice of the ovariectomized groupwere conducted with Bilateral oophorectomy,while fat beside ovaries were removed on mice of the sham-operation group.After operation 45 days,all mice were divided into 6 groups,as sham-operation group(isometric water),model group(isometric water),positive drug group(diethyl-stilbestrol,2 mg·kg~(-1)),high doses of Epmedin C group(20 mg·kg~(-1)),middle doses of Epmedin C group(10 mg·kg~(-1)),low doses of Epmedin C group(5 mg·kg~(-1)),with 14 mice in each group.Each group underwent intragastric administration once a day for 60 days.Then all mice were killed to weigh the uterus and calculate uterine factor.The content of estradiol in serum was determined by ELISA.Results:Compared with the sham-operation group,body weight of mice of model group were significantly increased,uterus weight and uterine factor and estradiol levels were significantly reduced,which suggested a significant difference.In comparison of the ovariectomized group,body weight of mice were relieved significantly and uterus weight and uterine factor and estradiol levels were increased significantly in all Epmedin C groups and diethylstilbestrol group(P0.05 or P0.01 ). Conclusion:Epmedin C has estrogen-like effects for ovariectomized mice.