The interaction between liver and intestine injury during hepatic ischemia/reperfusion in rats

Objective To investigate the interaction and its mechanism between liver injury and intestine injury during hepatic ischemia/reperfusion (I/R) in rats.Methods 24 SD rats,with the equal number of male and female,were allocated into three groups randomly:group 1(shunt group)?group 2 (non shunt group) and group 3(sham operation group ).The rats in group 1 underwent portal and inferior cava vein shunt before hepatic I/R(ischemia for 45 minutes and reperfusion for 2 hours).The rats in group 2 underwent the same procedure as that of group 1 except shunt procedure.All rats in group 3 were only subjected to laparotomy.Portal vein blood was obtained preoperation and post operation.Part of liver and ileum tissue were excised postoperatively for histological examination.Results The levels of ALT?AST?TNF? α and IL?1 have no significant difference among the three groups before operation,but after operation,the levels of the above measurement in group 1 and group 2 are higher than those of preoperation ( P 0.05).There were significant difference between group 1 and group 2 in postoperative measurements( P 0.05).As to group 3,no difference was found between preoperation and postoperation in all.There were significant differences among three groups in ICAM?1 expressed in liver.Conclusion This study demonstrated that not only ischemia itself does harm to liver,but the upgrated ICAM?1 in the liver by TNF? α and IL?1,which were induced by portal vein stasis,may aggravated the injury of the liver.Portal cava shunt may reduce this damage,and lead to a good liver function.