Expression of survivin and caspase-3 and its relation to the neruronal apoptosis after cerebral ischemia-reperfusion in rats

Objective To study the cerebral ischemia reperfusion injury Survivin and Caspase-3 expression and cell apoptosis.Methods42 adult male SD rats weighing 250～280 g, were randomly divided into control group(n = 3), sham operation group(n =3), ischemia-reperfusion 6 h, 12 h, 1 d, 2 d, 3 d, 6 d group(n =6). Prepared by the filament 90 minutes of middle cerebral artery occlusion and reperfusion model was observed by immunohistochemistry Survivin and Caspase-3 expression, TUNEL to detect apoptosis. ResultsIn the control group and the sham group no Survivin and Caspase-3 expression seen in apoptotic cells from 0 to 2, ischemia-reperfusion 6 h, displayed in the ischemic cortex, striatum and hippocampus subcortical Survivin and Caspase-3 increased expression, and apoptosis of cells increased, 12 h 1 d none of the three continued to increase, 2 d Caspase-3 expression and apoptosis peak, 3～ 6 d downward trend, while the expression of Survivin 3 d peak, continuing to 6 d a downward trend.Conclusion Focal cerebral ischemia and reperfusion injury induced by Survivin and abnormal expression of Caspase-3, and is closely related with cell apoptosis.