2008•Jiepou kexue jinzhanRequires access

TLR4 is Involved in the Apoptosis of Hippocampus Cells in Cerebral Ischemia-reperfusion Mice

Fang Xiu-bin

Abstract

Objective To study the effect of Toll-like receptor 4(TLR4) on the apoptosis in cerebral ischemia- reperfusion(I/R) mice. Methods 90 kun-ming mice were randomly divided into sham group, ischemia-reperfusion group and TLR4-blocked group in different time points of reperfusion (6h,12h, 24h, 48h and 72h). The expression of caspase-3 was studied by immunohistochemistry and Western blot, the apoptotic hippocampus cells were stained by TUNEL. Results Both the expression of caspase-3 and the number of apoptosis cells increased significantly in I/R group than in sham group(p 0.01), however the expression of caspase-3 and the number of apoptosis cells decreased in TLR4-blocked group compared with I/R group (p 0.01). Conclusion Blocking TLR-4 can decrease the cells apoptosis caused by ischemia-reperfusion.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Apoptosis, TUNEL assay, Ischemia, TLR4, Western blot, Hippocampus, Immunohistochemistry, Reperfusion injury