Application of EST-SSR Molecular Markers in Tea Varieties Identification

EST-SSR loci were screened from EST sequences of Longjing 43 in the NCBI public database,65 pairs of EST-SSR primers were designed using Primer 5.0 software.The genomic DNA of tea varieties Hubei No.1 tea,Longjing 43,Longjing groups,Yunkang No.10,Xueya 100 and Aifeng were extracted and used as a template to select EST-SSR primers.The results showed that targeted bands of all the 65 pairs of primers were amplified,the amplification rate was 100%.4 pairs of EST-SSR primers showed polymorphism in the 6 samples,could be used for identification of the 6 tea varieties.