Screening of Tea Polymorphic Primers of SSR Molecular Markers

In this study, 60 pairs of primers of tea SSR molecular markers were screened with the DNA templates of 12 new lines from FI natural hybrids of national tea cultivar Dangui. Electrophoresis of PCR products were found that about 12 pairs of SSR primers had ideal results with clear bands and rich polymorphism. The remaining 48 pairs of SSR primers were without polymorphic bands or with the vague bands can not be statistics, or the absence of any amplified bands. 59 new lines from FI natural hybrids of national tea cultivar Dangui were amplified with two pairs of primers of D02 and D08 randomly selected from the 12 pairs of polymorphic primers. Electrophoresis of PCR products can get clear bands and rich polymorphism gel figure, which illustrating 12 pairs of polymorphic SSR markers can be used for genetic diversity or phylogenetic relationships of molecular biology experiments and so on.