Establishment cervical cancer cell line with GFP expressing using replication incompetent lentiviral vectors

Objectives To establish a green fluorescent protein (GFP) stably expressing human cervical cancer cell line using replication incompetent and to investigate the expression of GFP in the cells.Methods Vector plasmid pGC-FU containing GFP gene as a target gene,package construct pHelper 1.0 and an envelope plasmid pHelper 1.0 were co-transfected into 293 T packaging cell line using Ca-P co-precipitation. The viruses harvested from the supernatant were used to transfect cervical cancer cells. Then the cells that strongly expressed GFP was selected with FACS. Results A stable GFP-expressing cell line S3 was established,in this cell line more than 99 % of the cells expressed GFP.There is no significant difference between Siha and S3 in its growth curves,cell cycle distributions,the quantity of expressing HPV-16E7 and tumorigenic abilities in nude mice. Conclusion These researches showed that the lentiviral vector was efficient to establish human GFP-expressing cervical cancer cell lines-S3,which could facilitate the research on gene regulation of cervical carcinoma.