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2004•Unpublished venueRequires access

Specific suppression of GFP gene expression by siRNA of GFP in lung carcinoma cell A549

Wei Han

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Abstract

Objective:It was designed to investigate whether small interfering RNA (siRNA) of green fluorescence protein (GFP) can inhibit the expression of GFP in lung carcinoma cell A549.Methods:A plasmid included mU6 promoter and siRNA of GFP was designed and constructed. The plasmid was transfected into lung carcinoma cell A549. GFP-siRNA effect of interference to GFP is observed and evaluate in A549 cell. Results: GFP-RNAi can inhibit specific expression of GFP gene in lung carcinoma A549 cell line and mU6 plasmid, no-related-siRNA have no such specific effect.Conclusion: These observations may open a path forward the use of siRNA as a new tool for researching gene function in cancer cell line.

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What this paper is about

Objective:It was designed to investigate whether small interfering RNA (siRNA) of green fluorescence protein (GFP) can inhibit the expression of GFP in lung carcinoma cell A549.Methods:A plasmid included mU6 promoter and siRNA of GFP was designed and constructed. The plasmid was transfected into lung carcinoma cell A549. GFP-siRNA effect of interference to GFP is observed and evaluate in A549 cell. Results: GFP-RNAi can inhibit specific expression of GFP gene in lung carcinoma A549 cell line and mU6 plasmid, no-related-siRNA have no such specific effect.Conclusion: These observations may open a path forward the use of siRNA as a new tool for researching gene function in cancer cell line.

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Available abstract

Objective:It was designed to investigate whether small interfering RNA (siRNA) of green fluorescence protein (GFP) can inhibit the expression of GFP in lung carcinoma cell A549.Methods:A plasmid included mU6 promoter and siRNA of GFP was designed and constructed. The plasmid was transfected into lung carcinoma cell A549. GFP-siRNA effect of interference to GFP is observed and evaluate in A549 cell. Results: GFP-RNAi can inhibit specific expression of GFP gene in lung carcinoma A549 cell line and mU6 plasmid, no-related-siRNA have no such specific effect.Conclusion: These observations may open a path forward the use of siRNA as a new tool for researching gene function in cancer cell line.

Key concepts: Green fluorescent protein, Transfection, A549 cell, RNA interference, Small interfering RNA, Molecular biology, Plasmid, Cell culture

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