Role of monocyte chemotactic protein-1 in acute lung injury during acute pancreatitis

[Objective] To investigate the role of Monocyte chemotactic protein-1(MCP-1)in the pathogenesis of acute lung injury(ALI)during acute pancreatitis(AP).[Methods] AP model was induced by intraperitoneal injection of cerulein or retrograde infusion of 5% sodium taurocholate into the bili-pancreatic duct in SD rats.Eighty-four SD rats were randomly divided into four groups:cerulein group,saline control group,sodium taurocholate group and operational control group.Expression of pulmonary MCP-1 protein and MCP-1 mRNA were detected by immunohistochemistry and semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR)at different time point in every group,respectively.[Results] No significant difference was found between cerulein group and saline control group at different time point.Expression of pulmonary MCP-1 proteins and MCP-1 mRNA in sodium taurocholate group 6 hours after operation were significantly higher than those of operational control group(P 0.01).Expression of MCP-1 was gradually up-regulated and correlated with the histological change of lung.[Conclusions] MCP-1 may play an important role in the pathogenesis of ALI during AP.