Genetic detection of Rifampin resistant in Mycobacterium tuberculosis

Eighty eight strains of clinical isolated Mycobacterium tuberculosis were analysed by PCR SSCP,with the drug susceptibility test as contral group.The objective DNA fragments were occured in eighty eight strains of clinical isolated Mycobacterium tuberculosis, but it not occured in ten strains of Mycobacterium non tuberculosis and in eight strains of non Mycobacterium. The primer used for amplify the 215bp of rpoB gene are specificity for Mycobacterium tuberculosis complex. Among the sixty eight rifampin resistant isolates,characterization of SSCP in sixty isolates of Mycobacterium tuberculosis are different from that in standard strain of M.tuberculosis. There are no difference between the twenty susceptible strains and the standard strain.In compare with the conventional susceptibility testing methods,the sensitivity and specificity of PCR SSCP for detection of rifampin resistance in sixty eight strains of M.tuberculosis was 88% and 100%,respectively.Using specific primer to amplify the fragment of rpoB,it can not only confirm the pathogen of M.tuberculosis, but can easily and rapidly detect rifampin resistance of M.tuberculosis by the PCR SSCP technique.