Rapid technique in detection γpoB gene mutation in mycobacterium tuberculosis by PCR-SSCP

Objective: To evaluate rifampin resistant Mycobacterium tuberculosis by PCR SSCP technique to detect γpoB gene mutation in mycobacterium tuberculosis of sputums.Methods: Fifty clinicae isolates of rifampin resistant and multidruy rdsistant mycobacterium tuberculosis,twelve sensitive strains, thirty five rifampin resistant strains from sputum specimens of patients with pulmonary tuberculosis, were analyzed by PCR SSCP technique, with H37Rv reference as control group.Results: The characteristics of PCR SSCP graph in fifty strains of rifampin resistant were different from H37Rv except for five strains.In comparison with susceptibility testing methods, the sensitivity and specificity were 90% and 100% respectively.Twelve sensitive strains were same as H37Rv.In thirty five strain from sputum spcimens of patients with pulmonary tuberculosis, twenty one strains of them were positive by PCR.The characteristic of SSCP graph in fifteen of them were different from H37Rv,and the characteristics of PCR SSCPgraph in twenty eight of them were different from H37Rv,the positivity was 80%.Conclusion: The PCR SSCP can easily and rapidly detect rifampin resistance of mycobacterium tuberculosis,the technigue might be used for rapidly detect mycobacterium tuberculosis rifampin resistance in clinical specimen directly.