The effect of fasudil on activation of p38MAPK in lung tissue of acute lung injury rats induced by lipopolysaccharide

Objective To investigate the potential effects of fasudil on phosphorylate activation of p38MAPK in lung tissue of acute lung injury(ALI)rats induced by lipopolysaccharide.Methods A total of 45 male SD rats were randomly divided into three groups:NS group(n=15),LPS group(n=15) and FAS group(n=15).ALI was induced by injecting LPS intravenously(5 mg/kg) in LPS group and FAS group.The FAS group was intraperitoneal injected with fasudil(10 mg/kg) thirty minutes before LPS injection.At 3 h,6 h,12 h after LPS injection,the pathomorphism changes of lung tissue were observed,and the expressions of p-p38MAPK protein in lung tissue were examined by immunohistoehemistry and Western Blot in all the rats.Results The pulmonary expressions of p-p38MAPK protein increased obviously in LPS group(P0.05).Meanwhile,lower levels of p-p38MAPK protein were found in FAS group than those in LPS group at all time points(P0.05).The pathological changes were more obvious in LPS group than those in FAS group.Conclusion Fasudil could attenuate the degree of acute lung injury of rats induced by LPS,which may be related to its effect in inhibiting the phosphorylate activation of p38MAPK in lung tissue.