The Protective effect of fasudil on acute lung injury induced by lipopolysaccharide in rats

Objective To explore the potential protective effect of fasudil on acute lung injury(ALI) induced by lipopolysaccharide in rats.Methods A total of 45 male SD rats were randomly divided into three groups:control group(NS group n=15),lipopolysaccharide group(LPS group n=15) and fasudil treatment group(FAS group n=15).The all model was established by injecting LPS intravenously(5 mg/kg) in the LPS group and the FAS group.The FAS group was injected intraperitoneally with fasudil(10 mg/kg) thirty minutes before LPS injection.Tumor necrosis factor-a(TNF-a) and interleukin(IL) 1β(IL-1 beta) level in serum were measured through ELISA method,the blood gas analysis,lung tissue wet/dry weight ratio(W/D),lung tissue HE dyeing were tested in 3 h,6 h,12 h three time points after LPS injection.Results In comparison with the LPS group,the arterial partial pressure of oxygen(PaO2) was significantly increased(P0.05) and the ratio of lung tissue wet/dry weight(W/D),Lung Injury Score and the levels of TNF-a and IL-1β in serum were significantly decreased(P0.05) in the FAS group in 3 h,6 h,12 h three time points after LPS injection.Conclusion Fasudil has a beneficial effect on the LPS-induced acute lung injury of rats,which may be related to its effect in inhibiting the proinflammatory cytokines release of TNF-a and IL-1β.