Apoptosis of PC12 cell Induced by Amyloid Beta-peptide Fragment 25-35

Purpose To study the neurotoxicity of amyloid beta-peptide fragment 25-35 (Aβ 25-35)in cultured PC12 cells. Methods The way of Aβ 25-35 induced PC12 cells injury was analyzed by MTT metabolic rate examination, acridine orange fluorescence staining light microcopy, transmission electron microscopy and flow cytometry. Results Treatment of PC12 cells with Aβ 25-35 for 24 h caused a dose-dependent decrease in MTT metabolic rate examination, morphological apoptosis of PC12 cells could been seen under fluorescence microscope and electron microscope. respectively, proportions of apoptotic cells of control group, 20 μmol/L and 50 μmol/L Aβ 25-35 group were 0.08%±0.01%,14.8%±1.13%,25.9%± 2.34% by flow cytometry. Conclusions Aβ 25-35 injured PC12 cells mainly via apoptotic pathway.