Bioinformatics analysis of 5' regulatory region of the tight junction protein claudin-10 gene

Objective:To study transcription and regulation mechanisms of the tight junction protein claudin-10 gene,and to analyze its potential transcription factor binding sites.Methods:TATA-box,GC-box and CAAT-box were analyzed with Basic Local Alignment Search Tool(BLAST);promoter sites,CpP island and the potential transcription factor binding sites of 5 'regulatory region of claudin-10 gene were analyzed with on-line software of Neural Network Promoter Prediction,Promoter Scan,Promoter 2.0,EMBOSS,CpG Island Searcher,and TFSEARCH seperately.Results:In 5 'regulatory region of the claudin-10 gene,a CAAT-box,3 GC-box,but no TATA-box was found;4 promoter sites might be presented in claudin-10 gene;CpG island was 444 bp(1 700 bp-2 143 bp)or 834 bp(1 367 bp-2 200 bp)in length.On circumstances of scores of 85,90,and 95(or above),159,48 and 9 potential transcription factor binding sites were separately found.Conclusion:The study of promoter sites,potential transcription factor binding sites for claudin-10 gene with bioinformatics approach is of great significance,but final conclusion needs to be confirmed by further experiments.