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2010•Science Technology and EngineeringRequires access

Physicochemical Properties and Anti-tumor Activities in vitro of Angelica sinensis Polysaccharide APS-bII

Qibing Mei

Open publisher page 2 citations

Abstract

The polysaccharide APS-bII was isolated from Angelica sinensis(Oliv.) Diels through water extraction, deprotein, ethanol precipitation, and was further purified by DEAE-Sephadex A-25 and Sephadex G-100 column chromatography.The molecular weight was carried out with high-performance size exclusion chromatography (HPSEC).Sugar composition analysis and infrared spectrum were used to determine the structural features.The HPSEC result showed the APS-bII was a single homogeneous component and its molecular weight was 1.3×104 Da.The monosaccharide composition of APS-bII was glucose, galactose, xylose, arabinose.The results of the MTT assay indicated that AP-bII inhibited the proliferation of several human cancer cells in a concentration-dependent manner.

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What this paper is about

The polysaccharide APS-bII was isolated from Angelica sinensis(Oliv.) Diels through water extraction, deprotein, ethanol precipitation, and was further purified by DEAE-Sephadex A-25 and Sephadex G-100 column chromatography.The molecular weight was carried out with high-performance size exclusion chromatography (HPSEC).Sugar composition analysis and infrared spectrum were used to determine the structural features.The HPSEC result showed the APS-bII was a single homogeneous component and its molecular weight was 1.3×104 Da.The monosaccharide composition of APS-bII was glucose, galactose, xylose, arabinose.The results of the MTT assay indicated that AP-bII inhibited the proliferation of several human cancer cells in a concentration-dependent manner.

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Available abstract

The polysaccharide APS-bII was isolated from Angelica sinensis(Oliv.) Diels through water extraction, deprotein, ethanol precipitation, and was further purified by DEAE-Sephadex A-25 and Sephadex G-100 column chromatography.The molecular weight was carried out with high-performance size exclusion chromatography (HPSEC).Sugar composition analysis and infrared spectrum were used to determine the structural features.The HPSEC result showed the APS-bII was a single homogeneous component and its molecular weight was 1.3×104 Da.The monosaccharide composition of APS-bII was glucose, galactose, xylose, arabinose.The results of the MTT assay indicated that AP-bII inhibited the proliferation of several human cancer cells in a concentration-dependent manner.

Key concepts: Ethanol precipitation, Xylose, Chemistry, Chromatography, Polysaccharide, Galactose, Sephadex, Size-exclusion chromatography

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Physicochemical Properties and Anti-tumor Activities in vitro of Angelica sinensis Polysaccharide APS-bII — Research Paper | ScholarLens