Experimental study on the effect of early interference of Fangxiaoyin Decoction on serum Interleukin-10 level in asthmatic mice

Objective To investigate the effects of Fangxiaoyin Decoction on the serum concentration of Interleukin-10(IL-10) and eosinophil of bronchoalveolar lavage fluid in asthmatic mice after early interference treatment.Methods Sixty healthy Kunming mice were randomly divided into six groups: Group A:normal control group,Group B:asthmatic model group,Group C:low-dose Fangxiaoyin decoction treatment group,Group D:medium-dose Fangxiaoyin decoction treatment group,Group E:high-dose Fangxiaoyin decoction treatment group,Group F:Budesonide(BUD)treatment group.The asthmatic mice model was stimulated with ovalumin.Fangxiaoyin decoction treatment groups were given from the first day sensitization by low-dose(containing crude drug 0.5 g/mL),medium dose(containing crude drug 1.5 g/mL),high-dose(containing crude drug 2.5 g/mL),once a day,each 0.4 mL,for a total of 27 days.At the 24 h after the final stimulation(28 days),the mice were executed.Detect IL-10 by ELISA.And then,detect the percentage of EOS of BALF by microscope.Results The percentage of EOS of BALF in group B was significantly higher than group A(P0.01).The concentration of IL-10 in group B was significantly lower than group A(P0.01).Group C,D and E the concentrations of IL-10 were significantly increased.There was significant difference between groups C,D,E and B(P0.01) on the concentrations of IL-10,and there was no significant difference between groupC,D,E and F(P0.05).In group C,D,E and F EOS% of BALFwas decreased(P0.01),there being no significant difference among the groups(P0.05).Conclusion Pathological state of asthmatic mice can be greatly improved after early interference treatment by Fangxiaoyin Decoction and BUD.The mechanism may be to increase IL-10 and decrease EOS% of BALF.