Protection of potassium channel antagonist tetraethylammonium against rat′s neuronal death induced by oxygen and glucose deprivation

Objective To investigate the effects of potassium channel antagonist on oxygen and glucose(OGD)-induced death of cultured hippocampal neurons.Methods After 12d′s cultue,Earle′s balanced salts(EBSS) took the place and then hippocampal neurons were put into the incubator with 37 ℃ and three anoxic gases(N2:CO2:O2= 94%:5%:1%).4 h later culture under normal condition vitro was done again.Meanwhile,different concentrations of potassium channel antagonist tetraethyla mmonium(TEA) were applied to the culture solution.After 24 h,the neuronal death was analyzed with MTT assay.Results Compared with the control group,in OGD group and the group with different concentrations of OGD+TEA,the survival rate of hippocampal neurons was obviously lower(P0.05);Compared with OGD group,in OGD+10 μmol·L-1 TEA group and OGD+500 μmol·L-1 TEA group,the survival rate of hippocampal neurons was obviously higher(P0.05);Compared with OGD group,in OGD+1 μmol·L-1 TEA group and OGD+5 mmol·L-1 TEA group,there was no statistical significance in the difference of the survival rate of hippocampal neurons(P0.05).Conclusion Potassium channel antagonist TEA can protect hippocampal neurons induced by oxygen and glucose deprivation from death,which indicates that enhanced activity of some kind potassium channel may get involved in the death of cultured hippocampal neurons induced by oxygen and glucose deprivation.