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2004•Journal of Pediatric PharmacyRequires access

Determination of astragaloside IV in Radix Astragali by HPLC-ELSD

QU Qiao-ling

Open publisher page 1 citations

Abstract

Objective: To develop a method to determine the content of astragalos ide IV in Radix Astragali by HPLC-ELSD. Methods: HPLC-ELSD was used to determine astragaloside IV in Radix Astragali. The separation was performed on C18 column a with a mixture of methanol-water (75:25) as mobile phase. The flow rate was 1. 0 ml/min. The temperature of drift tube for measurement was 40 C and the flow ra te of N2 was 2.0 Bar as well. Results: The average recovery is 98.73%, and RSD w as 1.87%. Conclusion: The method is precise in determining astragaloside IV in R adix Astragali.

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What this paper is about

Objective: To develop a method to determine the content of astragalos ide IV in Radix Astragali by HPLC-ELSD. Methods: HPLC-ELSD was used to determine astragaloside IV in Radix Astragali. The separation was performed on C18 column a with a mixture of methanol-water (75:25) as mobile phase. The flow rate was 1. 0 ml/min. The temperature of drift tube for measurement was 40 C and the flow ra te of N2 was 2.0 Bar as well. Results: The average recovery is 98.73%, and RSD w as 1.87%. Conclusion: The method is precise in determining astragaloside IV in R adix Astragali.

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Available abstract

Objective: To develop a method to determine the content of astragalos ide IV in Radix Astragali by HPLC-ELSD. Methods: HPLC-ELSD was used to determine astragaloside IV in Radix Astragali. The separation was performed on C18 column a with a mixture of methanol-water (75:25) as mobile phase. The flow rate was 1. 0 ml/min. The temperature of drift tube for measurement was 40 C and the flow ra te of N2 was 2.0 Bar as well. Results: The average recovery is 98.73%, and RSD w as 1.87%. Conclusion: The method is precise in determining astragaloside IV in R adix Astragali.

Key concepts: Drift tube, Astragaloside, Radix (gastropod), Chromatography detector, Chromatography, High-performance liquid chromatography, Chemistry, Biology

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