Established genescan technique of diversity gene of T cell antigen receptor CDR3 for assaying clonality of human TCR αβ T cell

[Objective] To establish genescan technique of diversity gene of T cell antigen receptor(TCR) complementary-determining region 3(CDR3) for assaying clonality of human TCR αβ T cell.[Methods] We took Jurkat cell and Raji cell as type strain and negative control respectively,and amplified 34 TCR α chain variable gene(TCR Vα) and TCR 26 β chain variable gene(TCR Vβ) of 5 healthy individuals peripheral blood T cell by RT-PCR.Productions of RT-PCR were assayed by genescan technique to determine clonality of TCR αβ T cell.[Results] Only mRNA genes of TCR Vα1.1 and TCR Vβ8 were found in Jurkat cells,no TCR Vα and TCR Vβ ge ne was found in Raji cells.Jurkat cell was monoclonal T cell.All the TCR Vα and TCR Vβ genes were expressed in 5 healthy individuals peripheral blood T cell.T cell were multiclonal.[Conclusion] Genescan technique of diversity gene of TCR CDR3 was stable and precise method for assaying clonality of human TCR αβ T cell.