Established genescan technique of diversity gene of T cell antigen receptor complementary-determining region 3 for assaying clonality of mouse TCR α β T cell

[Objective] To establish genescan technique of diversity gene of T cell antigen receptor(TCR) complementary-determining region 3(CDR3) for assaying clonality of mouse TCR αβ T cell. [Methods] 21 TCR α chain variable gene(TCR Vα) and 18 TCR β chain variable gene (TCR Vβ) of spleen T cell of C57BL/6H-2b, Balb/CH-2d and CB6F1(Balb/c×C57BL/6 F1H-2d/b) inbreeding line mouse were amplified by RT-PCR. Productions of RT-PCR were assayed by genescan technique to determine clonality of TCR αβ T cell. [Results] All the TCR Vα and TCR Vβ genes were expressed in spleen T cell of 3 kinds inbreeding line mouse. All the CDR3 repertoire showed Gaussian distribution, which suggested that T cell were multiclonal. The CDR3 of the TCR Vα and Vβ subfamily has the similar frequency of occurrence, but has the different polymorphism and length profiles.[Conclusion] Genescan technique of diversity gene of TCR CDR3 was stable and precise method for assaying clonality of mouse TCR αβ T cell.