Effects of Exogenous Salicylic Acid on Seed Germination and Seedling Growth Physiological Characteristics of Soybean under NaCl Stress
NI Xiangyi
Abstract
NI Xiangyi
Abstract
The effects of 0.2mmol·L-1 exogenous salicylic acid(SA)on seed germination,seedling morphology and biomass,membrane peroxidation,antioxidant enzymes in leaves of soybean(Glycine max L.) seedlings under 100mmol·L-1 NaCl stress were investigated by incubating with sand culture.The results showed that seed germination and seedling growth were significantly inhibited under NaCl stress;Relative electrolyte leakage and thiobarbituric acid reactive substance(TBARS)content were markedly increased; Activities of superoxide dismutase(SOD),catelase(CAT)and ascorbate peroxidase(APX)reduced constantly in leaves of soybean seedling under NaCl stress.While exogenous SA promoted seed germination and rootstock growth,increased biomass accumulation,reduced relative electrolyte leakage and TBARS content,improved activities of SOD,CAT and APX.The results suggested that NaCl stress inhibited significantly soybean seed germination and seedling growth,while certain concentration of exogenous SA couldenhance seed vigor and antioxidant enzymes activities,reduce the degree of membrane lipid peroxidation,alleviate damages by NaCl stress,and promote salt resistance of soybean seedlings under NaCl stress.
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The effects of 0.2mmol·L-1 exogenous salicylic acid(SA)on seed germination,seedling morphology and biomass,membrane peroxidation,antioxidant enzymes in leaves of soybean(Glycine max L.) seedlings under 100mmol·L-1 NaCl stress were investigated by incubating with sand culture.The results showed that seed germination and seedling growth were significantly inhibited under NaCl stress;Relative electrolyte leakage and thiobarbituric acid reactive substance(TBARS)content were markedly increased; Activities of superoxide dismutase(SOD),catelase(CAT)and ascorbate peroxidase(APX)reduced constantly in leaves of soybean seedling under NaCl stress.While exogenous SA promoted seed germination and rootstock growth,increased biomass accumulation,reduced relative electrolyte leakage and TBARS content,improved activities of SOD,CAT and APX.The results suggested that NaCl stress inhibited significantly soybean seed germination and seedling growth,while certain concentration of exogenous SA couldenhance seed vigor and antioxidant enzymes activities,reduce the degree of membrane lipid peroxidation,alleviate damages by NaCl stress,and promote salt resistance of soybean seedlings under NaCl stress.
Key concepts: Seedling, APX, Salicylic acid, TBARS, Germination, Chemistry, Horticulture, Superoxide dismutase