Establishment of an Immunosuppressive Animal Model Induced by Cyclophosphamide in Mice

Objective To establish immunosuppressive animal model and to study the assessment method for function food alleviating side effect of chemotherapy.[Methods] Different doses of cyclophosphamide(CP) were given to normal and tumor-bearing C57BL/6J mice every other day during 10 days.Immunological indices,liver and kidney functions were examined.[Results] 1.In 20 mg/kg bw group of normal mice,the white blood cells,antibody-producing cells and macrophage function declined and the level of serum AST increased significantly. In 50 mg/kg bw and 100 mg/kg bw groups of normal mice,NK cell activity descended and the levels of AST,ALT and BUN ascended significantly. 2.In all tumor-bearing mice groups,the white blood cells and macrophage function declined significantly. In 50 mg/kg bw and 100 mg/kg bw groups of tumor-bearing mice,spleen index,NK cell activity and antibody-producing cell function descended and the levels of AST,ALT and BUN ascended significantly. 3.After injection of 60mg/kg bw of CP,the levels of IL-2 and TNF-α in normal mice and the level of IL-2 in tumor-bearing mice decreased significantly. [Conclusion] Immunosuppressive animal model has been established by intraperitoneal injection of CP at the doses of 20-50 mg/kg bw to both normal mice and tumor-bearing mice.