Immunol function of palatin tonsil B lymphcyte

Objective:To study cellular immune function of palatine tonsil B lymph cell.Method:The phenotype of palatine tonsil cells(PTC) and that of peripheral blood mononuclear cell(PBMC) were compared using fluorescence staining and flow cytometry(FCM) analysis,then immunomagnetic beads were used to separate CD3-cell in PTC and PBMC.The proliferation function of CD3-lymph cell of PTC and PBMC was tested after stimulized by CD20mAb.Result:FCM analysis founding that 71.2% PTC express CD20 with higher mean fluorescence intensity,MFI,compared to the 15.5% in PBMC.There's no significant difference between the proliferation of PTC and PBMC B lymph cell.Conclusion:CD20 expression is different in PTC and PBMC,but corresponding function is still unknown.