Comparative analysis of the expression of CXCR5 and IL-21 on CD8~+ T cells from tonsil and peripheral blood

Objective To evaluate the differences of CXCR5 and IL-21 expression on CD8+ T cells from tonsil and peripheral blood.Methods The tonsillar mononuclear cells and peripheral blood mononuclear cells(PBMCs) were isolated,then four color flow cytometry(FCM) analysis was used to compare the expression of CD69 and CXCR5 on CD8+ T cells from tonsillar mononuclear cells and PBMCs.The expression of IL-21 on CD8+ T cells was also tested after stimulated by PMA and Ionomycin.Results The expression of CD69 and CXCR5 was significantly higher on CD8+ T cells from tonsil compared with that on CD8+ T cells from PBMCs(P0.05).The expression of IL-21 was also detectable on CD8+ T cells from tonsil after stimulated by PMA and Ionomycin.In contrast,CD8+ T cells from PBMCs did not produce IL-21.Conclusion The phenotype and function of CD8+ T cells from tonsil and PBMCs are different,the immunologic function of CD8+CXCR5+ and CD8+IL-21+ T cells from tonsil need to be studied in depth.