Antiproliferative Effect of Nolatrexed Dihydrochloride on Cell Line S-180 in vitro and in vivo

The anticancer effect of nolatrexed dihydrochloride on mouse cancer cell line S-180 in vitro and in vivo and the antiproliferative effect on normal human embryo kidney cell HEK293 were studied.The inhibition rate of the compound on the cultured cancer cell line S-180 and human embryo kidney cell HEK293 were assayed by MTT method. Antineoplasm effect on the mice with S-180 ascitic type of carcinoma and solid tumor were evaluated by survival time escalation and the weight reduction of the tumor tissue respectively. Result:The antiproliferative effect of nolatrexed dihydrochloride on cancer cell and normal cell were selective in vitro. The inhibitory potency of the compound on cancer cell S-180 was more powerful than that on the normal cell HEK293 (P0.01). The mean survival time of the mice with ascitic type of carcinoma was increased significantly when treated with nolatrexed dihydrochloride and fluorouracil (10?mg/kg) comparing to that of the untreated mice (P0.01). The mean survival time of high (100?mg/kg) and moderate (75?mg/kg) dosage of nolatrexed dihydrochloride treated groups were much longer than that of fluorouracil treated group (P0.05).So the antiproliferative effect of nolatrexed dihydrochloride on cancer cell line and normal cell was selective in vitro; the anticancer effect of nolatrexed dihydrochloride on the mice with S-180 ascitic type of carcinoma or solid tumor was obvious.