Inhibition of artesunate to mice cervical cancer U14 cell in vivo and in vitro

Objective To investigate the inhibitory effects of artesunate on cervical cancer in vivo and vitro.Methods The in vitro effect of different concentrations of artesunate on U14 cell was studied through the method of MTT.U14 cervical cancer mouse model with ascitic or solid tumor were constructed.The ascitic tumor bearing mice and solid tumor bearing mice were treated with artesunate in the concentrations of 200 mg/kg,100 mg/kg,50 mg/kg plus 2 mg/kg of cisplatin(DDP),and 50 mg/kg of artesunate plus 1 mg/kg of DDP respectively for a week;the control mice was treated with 0.9%NaCl.After treatment,the weight of the tumor and the survival time of the mice were observed to evaluate the growth inhibitory effect of artesunate.Results The in vitro experiment indicated that artesunate had growth inhibitory effect on U14 tumor cell with the fifty-percent inhibitory concentration(IC50) of 62.77?g/ml;The in vivo experiment showed that artesunate inhibited the growth of both solid tumor and ascitic tumor.With different concentrations of artesunate mentioned above,the tumor inhibition rates were 52.59%,44.44%,31.84%,61.45% and 51.85% respectively in solid tumor bearing mice and the rate of prolonged survival were 76.7%,45.0%,31.7%,100% and 93.3% respectively in ascitic tumor bearing mice.Conclusion Our in vitro and in vivo studies suggest the antitumor effect of artesunate on U14 tumor cell.