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2012•Acta Pratacultural ScienceRequires access

Comparison of SRAP and SSR markers for hybrid identification of switchgrass(Panicum virgatum)

Jiang Xiao-yang

Open publisher page 1 citations

Abstract

To provide a scientific basis for hybrid identification of switchgrass(Panicum virgatum) and cross-pollinating plants,we compared the identification efficiency and accuracy of SRAP and SSR markers.Switchgrass hybrid individuals(165) and their parental lines of switchgrass were tested by both methods.Compared to SRAP markers,the codominant SSR technique was more efficient.One pair of SSR primers(primer 23) detected all hybrids effectively and the percentage of polymorphic bands was 100%.SRAP required six primers to identify hybrids.The percentage of polymorphic bands of SRAP was 40% lower than that of SSR.

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What this paper is about

To provide a scientific basis for hybrid identification of switchgrass(Panicum virgatum) and cross-pollinating plants,we compared the identification efficiency and accuracy of SRAP and SSR markers.Switchgrass hybrid individuals(165) and their parental lines of switchgrass were tested by both methods.Compared to SRAP markers,the codominant SSR technique was more efficient.One pair of SSR primers(primer 23) detected all hybrids effectively and the percentage of polymorphic bands was 100%.SRAP required six primers to identify hybrids.The percentage of polymorphic bands of SRAP was 40% lower than that of SSR.

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Available abstract

To provide a scientific basis for hybrid identification of switchgrass(Panicum virgatum) and cross-pollinating plants,we compared the identification efficiency and accuracy of SRAP and SSR markers.Switchgrass hybrid individuals(165) and their parental lines of switchgrass were tested by both methods.Compared to SRAP markers,the codominant SSR technique was more efficient.One pair of SSR primers(primer 23) detected all hybrids effectively and the percentage of polymorphic bands was 100%.SRAP required six primers to identify hybrids.The percentage of polymorphic bands of SRAP was 40% lower than that of SSR.

Key concepts: Panicum virgatum, Hybrid, Biology, Panicum, Identification (biology), Primer (cosmetics), Biotechnology, Agronomy

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