Comparison of SSR and SRAP Marker for Varieties Identification in Maize(Zea mays L.)

SSR marker is usually the prior candidate of molecular marker for seed identification in maize.Unfortunately,some problems still exist in hybrids purity identification.Consequently,supplementary markers are still necessary to be explored to facilitate varieties identification besides SSR marker.In this study,SSR and SRAP markers were performed in 19 maize varieties and 8 samples of Lainong 15.The discriminative power of the markers,genetic relationship and hybrids purity revealed by SSR and SRAP markers were compared and analyzed.Comparing to SSR markers,SRAP markers displayed more amplified alleles per primer-pair,higher PIC value,and higher discriminative power.For the 19 maize varieties,genetic distance estimated based on SSR and SRAP data were significantly correlated with each other,and similar clustering results were achieved.Specific primers of SSR and SRAP with complementary pattern were used to detect hybrid purity of Lainong 15.The hybrid purity identified by SRAP marker was more vicinal to field test than that by SSR marker.In conclusion,SRAP markers could be applied in maize identification as a useful supplement of SSR,especially in hybrid purity identification.