RAPD analysis of Rubus spp.

The genetic diversity of twenty-six populations including thirteen cultivars of blackberry and raspberry and five wild species of Rubus L.were analyzed by RAPD technique.The results show that 131 bands are amplified by fifteen oligonucleotide random primers,and there are 118 polymorphic bands that account for 90% of the total amplified bands,which indicated that there are abundant genetic diversity among species and cultivars of Rubus.The twenty-six populations can be divided into four groups according to the cluster analysis result of RAPD bands.Group A includes R.alceaefolius Poir.,R.corchorifolius L.,R.coreanus Miq.,R.chingii Hu,‘Willamette',‘Tulameen',‘Taylor',‘Kivigold',‘Bristol',No.7 of ‘Boysen',‘Brazos',‘Brazos' seedlings,‘Kiowa',‘Shawnee' and 'Blackbutte';group B includes Nos.1-6 of ‘Boysen',‘Marion' and ‘Choctaw';group C includes 'Hull' and 'Chester';group D only includes R.hirsutus Thunb.It is suggested that this cluster result is basic coincidence with the morphological taxonomic results of Rubus L.