Clinical Significance of Detection of bcr/abl Fusion Gene in theDiagnosis and Minimal Residual Disease of Chronic Myeloid Leukemia

Objective To explore the significance of bcr/abl fusion gene in the diagnosis and the minimal residue disease monitoring CML.Methods The bcr/abl fusion gene mRNA levels of 337 CML patients were detected by RT-PCR.632 samples were detected by the same method for monitoring the MRD of all patients.26 patients,22 patients and 289 patients weredivided into three different groups(allo-HSCT,STI571 and other therapy regimens)respectively.Results The bcr/abl fusion genes were checked out in 325 patients of total 337 CML patients,and the positive ratio was 96.4%.Three transcripts,b3a2,b2a2 and b3a3 were found in all patients,the proportionwas 58.5%,41.2% and 0.3% respectively.88.5%,59.1% and 4.1% patients in three different groups had negative bcr/abl fusion gene results after variable regimens respectively.The negative results were checked out in day 30 to day 201,median day 55 in the allo-HSCT group,and in day 90 to 365,median day 210 in theSTI571 group.Conclusion Detection and continuous monitoring of bcr/abl fusion gene have great significances in the diagnosis,the evaluation of treatment effect and the prognosis of CML patients.