Quantification of bcr/abl~(P190) fusion gene transcripts by real time quantitative PCR in patients with chronic myeloid leukemia

Objective To study the expression and its clinical relevance of bcr/abl~(P190) fusion gene transcripts in patients with chronic myeloid leukemia(CML).Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases.Results bcr/abl~(P190) transcripts were detected in 54(77.1%) cases.The median bcr/abl~(P190)level was 2.60(1.08~8.58) 10~(-3),which was not correlated with the sex,age,leukocyte counts,hemoglobin,platelets,and the types and expression level of bcr/abl~(P210)fusion transcripts.The white blood cells and platelets in bcr/abl~(P190)positive group were significantly lower than those in bcr/abl~(P190) negative group.There were no significant differences in bcr/abl~(P190) levels between the patients in blastic crisis and in chronic phase and between the patients with different blastic phenotypes.The bcr/abl~(P190) level was decreased in cases obtained complete cytogenetic remission.Conclusion The different types of bcr/abl splicers were present in CML patients in(different) phases and there may be a certain relevance of splicing and hematological parameters.