Evaluation of the new body fluid mode on the Sysmex XE-5000 for detecting of tumor cells in ascitic and pleural fluids

Objective To evaluate the capability for detecting of tumor cells in ascitic and pleural fluids by Sysmex XE-5000 hematology analyzer.Methods 91 EDTA-K2 ascitic and pleural fluids samples were counted by Sysmex XE-5000 hematology analyzer.Highly fluorescent body fluid cells percent(HF-BF%) and scatter diagram were observed in the samples to evaluate the capability of detection of tumor cells.SPSS11.5 and Excel were used for statistical analysis.Results The samples with tumor cells showed increased Highly fluorescent body fluid cells percent(HF-BF%) compared with benign samples.Bright band composed by dense cells whose distribution was located at the zone with high side fluorescence lights(SFL) and side scattered lights(SSC) in scatter diagram provided information about the possible presence of tumor cells.Conclusion Highly fluorescent body fluid cells percent(HF-BF%) and distinguishing feature of scatter diagram can provide screening information about the presence of tumor cells.This suggests that such increase should lead to a manual differential review and an extensive examination of slides to check the presence of abnormal cells.