Construction of cDNA Expression Library from Eggs of Haemaphysalis longicornis

【Objective】 cDNA expression library from eggs of Haemaphysalis longicornis was constructed for further screening and cloning of the potential candidate antigenic genes. 【Method】 Total RNA was isolated from eggs of H. longicornis, mRNA were purified, and cDNAs were synthesized and ligated to λSCREEN vector. The recombinant phage DNA was packaged by using Phagemark packaging extracts and then transfected to E. coli ER1647 to obtain the cDNA expression library. 【Result】The size of the primary cDNA library was 1.38×106 PFU with titer of the amplified cDNA library of 2×109 PFU·ml-1. A full length cDNA encoding follistatin-related protein was cloned from the cDNA library by PCR. 【Conclusion】The cDNA expression library from eggs of H. longicornis was successfully constructed.