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Construction of cDNA expression library of Haemaphysalis longicornis tick

Ning Jia

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Abstract

Total RNA and subsequently mRNA were isolated from salivary gland organs dissected from partially engorged Haemaphysalis longicornis ticks.A library of oligo(dT)-primed cDNA with added directional EcoR I/Hind III linkers was constructed and ligated to the EcoR I/Hind III arms of the λscreen vector.The recombinant phage DNA was packaged by using Phage Maker packaging extracts,resulting in a primary cDNA library with a size of 4.2×106 PFU.The library was proved to have good quality by all the control tests.

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What this paper is about

Total RNA and subsequently mRNA were isolated from salivary gland organs dissected from partially engorged Haemaphysalis longicornis ticks.A library of oligo(dT)-primed cDNA with added directional EcoR I/Hind III linkers was constructed and ligated to the EcoR I/Hind III arms of the λscreen vector.The recombinant phage DNA was packaged by using Phage Maker packaging extracts,resulting in a primary cDNA library with a size of 4.2×106 PFU.The library was proved to have good quality by all the control tests.

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Available abstract

Total RNA and subsequently mRNA were isolated from salivary gland organs dissected from partially engorged Haemaphysalis longicornis ticks.A library of oligo(dT)-primed cDNA with added directional EcoR I/Hind III linkers was constructed and ligated to the EcoR I/Hind III arms of the λscreen vector.The recombinant phage DNA was packaged by using Phage Maker packaging extracts,resulting in a primary cDNA library with a size of 4.2×106 PFU.The library was proved to have good quality by all the control tests.

Key concepts: Haemaphysalis longicornis, Complementary DNA, cDNA library, Biology, Recombinant DNA, Tick, Molecular biology, Vector (molecular biology)

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