Analysis of the Gene Expression Changes in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 Betected by Microarray Before and After Transfection with nm23-H1 Gene

To investigate the changes of gene expression in the human high-metastatic large cell lung cancer cell line L9981 transfected and untransfected with nm23-H1 gene.The L9981 and L9981-nm23-H1 cells were cultured in RPMI 1 640 medium supplemented with 10% calf serum.Total RNA of the L9981 and L9981-nm23-H1 cells were then extracted,purified and subject to reverse transcription into first-strand cDNA,labeled with Cy3/Cy5.Placenta DNA microarrays containing 14 000 DNA fragments were used to analyze the differential gene expression of L9981 and L9981-nm23-H1 cells. 1 156 genes (8.26%,1 156/14 000) were upregulated and 642 genes (4.59%,642/14 000) were downregulated in the nm23 transfected cell line,in-cluding many different types of genes,such as those responsible for signal transduction,oncogene and tumor suppression genes,metastatic-associated genes,apoptatic-related genes and DNA binding and transcription factor genes.These data suggested that the molecular mechanism by which nm23-H1 suppresses L9981 cells invasion and metastasis may be via regulating the expression of metastatic-related genes.