Analysis of p16 Gene Deletion and Mutation in Gastric Carcinoma

Objective To investigate the frequencies of homozygous deletion and mutation of P16 gene and to evaluate the clinic significance.Methods PCR technique was used to detect homozygous deletions of exon 1 and exon 2 of p16 gene and DNA sequencing technique was used to detect the genetic mutation.Results The homozygous deletion ratio of exon 1 and exon 2 of p16 gene in 30 gastric cancer tissues was 6 out of 30 cases and 3 out of 30 cases,respectively. Six of which belonged to stage Ⅲ, poorly differentiated cancer. In DNA sequencing, no mutation was found in all cases.Conclusion The abnormality of p16 gene is an important event in the progression of gastric cancer and may be associated with the development of gastric cancer.