The Expresstional Differences and Developmental Changes of bcl-2, P53 Gene in Early Embryos of Generic Hybrids of Chicken-Quail

【Objective】To investigate the influences of apoptotic factors bcl-2, P53 on early embryos of generic hybrids of chicken-quail. 【Method】The cross-bred eggs of quail (♀) acquired artificially inseminated by chicken(♂)-semen, hatched the eggs in one batch according to the standard condition of chicken, and collected the early living-embryos of 66, 72, 78, 84, 90, 96, 102, 108, 114 and 120 h at random. The method of RT-PCR was used to multiply PCR to identify and clarify the quail’s sex and embryo sex with primers of Wpkci and β-actin, then selected four embryos of female and male respectively at each period, taken β-actin for internal standard to determine the relative quantity of embryo bcl-2 and P53 mRNA.【Result】(1) The bcl-2 mRNA expression of 66-114 h of male embryos maintained lower level, declined on 96 h, then reached the initial level, rose distinctly on 120 h (P0.01) and reached the peak; the bcl-2 mRNA expression of 66-114 h of female embryos maintained lower level, rose distinctly on 114 h (P0.01), then maintained higher level and reached the peak on120 h; the female bcl-2 mRNA expression of 114 h was significantly higher than male of the same days (P0.05); (2) The patterns of P53 mRNA expression of male and female embryos were basically consistent. They were higher on 66 h, declined on 72 h (P0.05) and reached the lowest level, declined distinctly in male, but the whole differences were not significant in female, then reached and maintained the initial level. Compared the expression between different sexes, the differences were not significant.【Conclusion】At 114 h the expressional peak of bcl-2 mRNA of female embryos coincide with its P53?bcl-2 lowest expressional level; At 72 h P53 mRNA expressional quantity of male and female embryos were consistentwith their P53?bcl-2 expressional level, both were at low level. At 72, 114 h the bcl-2, P53 mRNA expression of early embryos of hybrids were abnormal.