On the Expression Differences between bcl-2 and p53 Genes after Sex Reverse in Chicken,Quail and Hybrid Embryos Induced by Formestane and 17β-estradiol

In this study,using exogenous hormones Formestane and 17β-estradiol,sex reversal experiments were conducted among chicken,quail and their hybrids embryos,and live embryos of 6 time points(72,96,120,144,168 and 192 h) were then sampled correspondingly.By quantitative PCR,we analyzed expressional differences of bcl-2 and p53 genes of different embryos at different stages after hormone treatment.The results revealed that bcl-2 and p53 gene expression showed no significant difference between normal chicken and quail embryos within heterosexual groups,but compared with chicken and quail embryos,there was significantly difference in hybrid embryos(P0.05);At 72 h,p53/bcl-2 of male and female embryos of chicken and quail both appeared low gene expression,and meantime,the p53 mRNA expressional quantity fell to relative low point,which indicated that the most embryos' death might happen at this period;gene expressions of bcl-2 and p53 of the chicken and quail embryos injected with Formestane and 17-estradiol were quite different from normal chicken and quail embryos(P0.05).But hybrid embryos were all dead at 72 h after they were injected exogenous hormones.