2007•Unpublished venueRequires access
Comparison of two species diabetic rats animal model for EB permeability to the blood-retina barrier
Nai Liu
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Abstract
Objective To investigate the changes of two species diabetic rats animal model for Evans-Blue (EB) permeability to the blood-retina barrier.Methods After EB(45 mg·kg~ -1 )was injected through jugular vein,control group and 1 week,4 weeks and 16 weeks diabetic Sprague-Dawley (SD)and Brown-Norway(BN) rats were respectively detected for the content of EB in the retina.Results Retinal EB leakages of retina from control, group 1 week, 4 weeks, and 16 weeks for two species diabetic rats were (12.24±3.11)ng·mg~ -1 ,(21.68±4.41) ng·mg~ -1 ,(21.78±3.17 )ng·mg~ -1 ,(23.56±4.05 )ng·mg~ -1 and(13.22±3.59) ng·mg~ -1 ,(23.41±4.47 )ng·mg~ -1 ,(26.40±5.00) ng·mg~ -1 , (31.01±4.46) ng·mg~ -1 ,respectively.EB in diabetic rats was much higher than that in control group(P0.05).Compared the rats of two different breeds, EB of BN diabetic rats was much higher than that of SD rats(P0.05),which was detected in 1 week,4 weeks and 16 weeks,and there was no significant difference compared with the control group (P0.05).Conclusion By detecting EB, the change of blood-retinal barrier permeability of diabetic rats with retinal lesion in early stage can be exactly observed.Genetic background determines the changes of retinal permeability in diabetic rats induced by SIZ.