Changes in microvascular permeability of lung in experimental diabetic rats and its significance

Objective To observe the changes of microvascular permeability of lung in experimental diabetic rats, and to offer clinical morphologic data for diabetic pulmonary disease. Methods 60 male SD rats with(220 ±20) g were selected and injected with Streptozotocin(STZ) intraperitoneally at a dose of 65 mg/kg body weight to make experimental DM rat models. Then respectively, 12 of them were randomly selected at 0, 2, 4, 8, 12 weeks to measure their dry weight and wet weight of lung tissues. Also, the OD values were observed through spectrophotometer and the amount of fluorescence by a fluorescence microscope after the rats were injected Evans blue(EB) into tail vein. Another 12 rats were randomly selected as normal group. Results ①The difference of lung tissue wet-dry weight among normal group and 0 week group, 2 weeks group was not statistically significant(P 0.05). The lung tissue wet-dry weight in 4 weeks group, 8 weeks group, 12 weeks group were all higher than those in normal group, the differences were statistically significant(P 0.05). ②The difference of lung tissue EB content among normal group and 0 week group, 2 weeks group was not statistically significant(P 0.05). The lung tissue EB content in 4 weeks group, 8 weeks group, 12 weeks group were all higher than those in normal group, the differences were statistically significant(P 0.05). ③And expression strong red fluorescence was found in the lung tissue of 4 weeks, 8 weeks, 12 weeks group through the fluorescence microscopy. Conclusion Diabetes leads to the changes of lung microvascular permeability in rats.