fect of Kangxian Ruangan Granule on Proliferation of Hepatic Stellate Cells Induced by Platelet-derived Growth Factor

abstractjective:To investigate the effect of Kangxian Ruangan granule (KXR) on hepatic stellate cells(HSC) proliferation induced by platelet-derived growth factor ( PDGF) in vitro. Methods: Serum-free culture method was used. HSC were treated with KXR for 24 hours, and stimulated with PDGF-BB for 24 hours, and then cultured with the same concentration of KXR for 3 hours, stimulated with PDGF-BB for 5 minutes. The cells were collected and the proliferation of HSC was examined with cell counting method. The cell cycle was analyzed with flow cytometry. Results: Serum-free culture indicated KXR could attenuate HSC growth with dose-dependant tendency ( P 0. 01). Flow cytometry analysis indicated KXR could inhibit DNA synthesis of S and G2/M phases induced by PDGF, block HSC cell cycle beyond G1 phase, which existed dose-dependent relation ( P 0. 01). Conclusion: KXR could inhibit the proliferation of HSC induced by PDGF.