Analysis of the result of antinuclear antibody and its profile for 1725 patients

Objective To explore the clinic value and the extent consistent of antinuclear antibodies(ANA) and antinuclear antibody profile in the autoimmune diseases(AID).Methods Retrospective analysis of the result of ANA and ANA profile in sera of 1725 patients.ANA and anti-double-stranded DNA antibody were detected by indirect immunofluorescence(IIF),ANA profile were detected by Euronline(immunoblot assay).Results 768 ANA positive cases were found out of the 1725 patients,the positive rate being 44.52%.The main fluorescent type of ANA were nuclear particles pattern,nuclear homogenous pattern,nucleolar pattern.Of all the 1725 samples,782 cases is positive for ANA profile.The positive rate is 45.33%.Most of the antibodies appear together two or more positive joint,the most frequently occurred antibody was anti-SS-A,anti-Ro-52,anti-SS-B and anti-Histone.Conclusion ANA and ANA profile can supplement each other in diagnosing AID and increase the detection rate,which is of great clinic value to diagnostic classification,treatment,post-prediction judge and disease follow-up of AID.