In Vitro Culture of Onoclea sensibilis L.from Spores

With mature spores of Onoclea sensibilis as the explants,the effects of different kinds and concentrations of plant growth regulator combination in MS medium on spore germination,callus induction,bud differentiation and rooting were studied.The results showed that the most suitable medium for spore germination was 1/2MS+2% sucrose,and after 20 days of spore sowing on this medium,the germination rate was 55.7%.The maximum callus induction rate was 36% which obtained from medium of MS+0.5mg·L-1 KT+0.5mg·L-12,4-D.The form of callus was green granular;the induced rate of bud from granular callus was 49% in MS.And IAA can promote rooting rate effectively.