Effect of hyperthermia combined with Taxol on the proliferation and apoptosis of human larynx carcinoma Hep-2 cell lines

【Objective】To determine the effect of hyperthermia combined with Taxol on the proliferation and apoptosis of human larynx carcinoma Hep-2 cells in vitro.【Methods】Hep-2 cells were divided into an experimental group and a control group,and the experimental group was further divided into 0.1 μmol/L Taxol group,1.0 μmol/L Taxol group,10 μmol/L Taxol group and hyperthermia group,Hep-2 cells were treated with hyperthermia combined with Taxol Wright-Giemsa dying assay was used to observe the apoptosis morphology of Hep-2 cells.The proliferation of the cells treated with hyperthermia and Taxol was detected by the method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),colony formation test and Flow cy-tometry(FCM) was used to detect the apoptosis rate of lymphoma and the fluorescene density in the lympho-cytes.【Results】Compared with the same dosage of paclitaxel but different temperature,the Hep-2 cell prolif-eration rate in the group heating at 41℃ decreased significantly(P 0.05).The proliferation rate of Hep-2 cell in 39℃ and 43℃ groups decreased significantly compared with the 37℃ control group(P 0.05).Evident apoptosis morphological changes of Hep-2 cells were observed 48 hours ofter treatment with hyperthermia or Taxol,compared with hyperthermia or Taxol alone,hyperthermia combined with Taxol could increase the inhi-bition ratio sisnificantly(P 0.01),and the inhibition rate was related to the concentration and action time of Taxol(P 0.05),compared with hyperthermia or Taxol alone,hyperthermia combined with Taxol could increase the apoptosis rate Hep-2 cells with obvious difference(P 0.05).Conclusion of The reverse effect of hyper-thermia combined with Taxol 48 h on Hep-2 cells was determined by cell growth curve,the doubling time and the number of colony formation were calculated based test.Light and electronic microscopy was used to observe morphological changes of Hep-2 cell lines.The apoptotic rate of Hep-2 cell lines in both testing group and control group cells were analyzed by flow cytometry.The cells apoptosis rates were detected by flow cytometry(FCM).Result compared with Taxol or hyperthermia,the hyperthermiaat 41℃ for 1 hour com-bined with Taxol showed obvious inhibitory effect in Hep-2 cells(P 0.05).【Conclusion】Hyperthermia or Taxol alone or combined can inhibit the proliferation,induce cell apoptosis,hyperthermia combined with Taxol has synergistic anti-Hep-2 cells effect in vitro.Hyperthermia combined with Taxol can inhibit the prolifera-tion and apoptosis of Hep-2 cells in vitro and inhibit proliferation in vitro with synergistic effect.