Experimental study of radiosensitization effect of taxol on laryngeal squamous carcinoma Hep-2 cell lines

Objective To investigate the anti-tumor effect of taxol combined with accelerated radiation therapy and the effect of taxol on the cell cycle of Hep-2 cell lines,and to determine the mechanism of action of taxol combined with radiation in vitro.Methods ① The Hep-2 cell lines were divided into radiation group(radiation only),taxol combined with radiation groups(exposed to taxol for 12,18,24,30 h and then radiated) and radiation combined with taxol groups(radiated and then exposed to taxol for 12,18,24,30 h).The MTT assay was used to detect the growth inhibition rates of cell lines in all groups.② After Hep-2 cell lines were exposed to taxol for 12,18,24,30 h,cell cycle analyses were performed by FCM.Results ① The cell growth inhibition rates of taxol combined with radiation groups(averaged from 87.51% to 89.64%) were much higher than those of radiation combined with taxol groups(averaged from 57.84% to 64.34%) and radiation group(41.90%).The differences of cell growth inhibition rates were statistically significant(P0.01).② After exposed to taxol,the cells shifted from G0/G1 phases to G2/M phases depending on the exposure time. Apoptosis was induced after the cells were exposed to taxol for 30h.Conclusion Taxol is an active radiation sensitizer of Hep-2 cell lines.One of the mechanisms may be that it can accumulate the Hep-2 cell lines in G2/M phases in which cells are more sensitive to radiation.