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Study on the expression of PoIFN-γ in the recombinant Pichia pastoris and the biological characteristics

Cao Han-wei

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Abstract

Porcine interferon-gamma(PoIFN-γ) cDNA was cloned by RT-PCR from porcine lymphocytes in peripheral blood.The recombinant plasmid(pPICZα-PoIFN-γ) encoding PoIFN-γ gene was constructed and transferred into Pichia pastoris X33 strain.A high level expression of PoIFN-γ in the recombinant Pichia pastoris was detected by SDS-PAGE analysis and Western-blot assay.The recombinant PoIFN-γ can protect the porcine kidney cells(PK-15) from the attack of vesicular stomatitis virus in vitro and significantly enhanced the immune response of pseudorabies virus vaccine in pigs.

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What this paper is about

Porcine interferon-gamma(PoIFN-γ) cDNA was cloned by RT-PCR from porcine lymphocytes in peripheral blood.The recombinant plasmid(pPICZα-PoIFN-γ) encoding PoIFN-γ gene was constructed and transferred into Pichia pastoris X33 strain.A high level expression of PoIFN-γ in the recombinant Pichia pastoris was detected by SDS-PAGE analysis and Western-blot assay.The recombinant PoIFN-γ can protect the porcine kidney cells(PK-15) from the attack of vesicular stomatitis virus in vitro and significantly enhanced the immune response of pseudorabies virus vaccine in pigs.

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Available abstract

Porcine interferon-gamma(PoIFN-γ) cDNA was cloned by RT-PCR from porcine lymphocytes in peripheral blood.The recombinant plasmid(pPICZα-PoIFN-γ) encoding PoIFN-γ gene was constructed and transferred into Pichia pastoris X33 strain.A high level expression of PoIFN-γ in the recombinant Pichia pastoris was detected by SDS-PAGE analysis and Western-blot assay.The recombinant PoIFN-γ can protect the porcine kidney cells(PK-15) from the attack of vesicular stomatitis virus in vitro and significantly enhanced the immune response of pseudorabies virus vaccine in pigs.

Key concepts: Pichia pastoris, Recombinant DNA, Biology, Molecular biology, Complementary DNA, Vesicular stomatitis virus, Plasmid, Virology

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