The effect on cardiac function Bone marrow stromal stem cells transplantation via coronary artery after acute myocardial infarction

Objective:To investigate the capability of autologous bone marrow stromal stem cells for repairing of damaged myocardium through the establishment of acute myocardial infarction model and the inducement of bone marrow stromal stem cells.Methods:Twelve hybrid dogs were randomly separated into two groups including the control group(n=6) and the cell transplantation group(n=6) according to the date of surgical operation.Anterior descending coronary arteries of hybrid dogs were ligated to establish the models of acute myocardial infarction.Autologous bone marrow stromal stem cells induced and cultured in vitro were labeled and transplanted into myocardium via coronary artery 1~2 hours after acute myocardial infarction in the cell transplantation group,and the same volume of culture media was transplanted into those in the control group.Echocardiography were applied seven days and eleven weeks after operation.Then the hybrid dogs were executed and the hearts were obtained for immunohistochemical analysis.Results:Compared with control group,LVEF in cell transplantation group was significangly higher than that of control group(P0.05) 11 weeks after MSSCs implantation,the improvement of myocardial segment in cell transplantation group was higher than that of control group(P0.05).In cell transplantation group,BrdU and Troponin I and Connexin 43 positive stain were tested with immunohistochemistry,then those were negative in control group.Conclusions:Bone marrow stromal stem cells survived and propagated in survival myocardium after transplantation via coronary artery.These cells can improve the blood perfusion and metabolism of survival myocardium,improving LVEF and myocardial function.