2004•Prevention and Treatment of Cardio-Cerebral-Vascular DiseaseRequires access
Observation of Autologous Canine Bone Marrow Stromal Stem Cells Transplantation via Coronary Artery for Myocardial Infarction
Zonggui Wu
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Abstract
Objective To observe the outcome of transplantation of autologous bone marrow stromal stem cells into myocardium via coronary artery in adult canine.Methods Acute myocardial infarction was induced by ligating the left anterior descending coronary arteries in adult canine. Bone marrow stromal cells aspirated from bone marrow were cultured and induced with 5_aza for 4 weeks. In experimental group cultured cells were transplanted into myocardium via coronary artery and in control group same volume of culture media was injected 4 weeks after myocardial infarction. Pathology, immunohistochemical and electron microscopy examination of heart specimen were carried out 4 weeks after cell transplantation. Results Implanted cells were viable in myocardial scar tissue in experimental group. Immunohistochemical study showed that Desmin and Tropponin I were positive in implanted cells. By electron microscopy,heterogeneous cells with myofilament structure were fownd in scar tissues of myocardium. However, there were no heterogeneous cells in myocardial scar tissues in control group. Immunohistochemical study showed that Desmin was negative and Troponin I was positive in myocardium in control group. Electron microscopy showed no heterogeneous cells in scar tissues of myocardium. Conclusions Adult canine marrow stromal stem cells survived and differentiated into myogenic cells in myocardial scar tissues after transplantation via coronary artery.